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. 2014 Apr 9;289(23):16270–16277. doi: 10.1074/jbc.M114.569707

FIGURE 3.

FIGURE 3.

Inhibition of RNase H activity of HIV-1 RT and E. coli RNase H by GSK5750 and β-thujaplicinol. A, a chimeric DNA-RNA/DNA substrate was used in the assay, which mimics the (−)-strand primer removal reaction. The primary RNase H cleavage occurs at the DNA/RNA junction +1, as indicated. * indicates 5′- radiolabeled primer. B, comparative effects of GSK5750 and β-thujaplicinol on the RNase H activity of HIV-1 RT and E. coli RNase H on the chimeric substrate shown in A. C, results from B for the HIV-1 RT primary (○) and secondary (■) cuts are shown graphically. IC50 values for HIV-1 RT secondary cleavages are 0.33 ± 0.11 μm and 3.8 ± 0.65 μm for GSK5750 and β-thujaplicinol, respectively.