FIGURE 4.
Inhibition of collagen binding of AAPP by antibody. AAPPex1–4 was serially diluted and incubated for 1 h with indicated doses of the 8H7 Fab (A) and whole IgG (B) and then applied into the collagen-coated plates. Plate-bound AAPP was detected by anti-His antibody conjugated with horseradish peroxidase. C, 5 or 2.5 nm AAPPex1–4 was incubated with serially diluted 8H7 Fab and then applied into the collagen-coated plates. Plate-bound AAPP was detected by anti-His antibody conjugated with HRP. D, AAPPex1–4 was serially diluted and incubated for 1 h with 1000 nm control mouse IgG and then applied into the collagen-coated plates. Plate-bound AAPP was detected by anti-His antibody conjugated with HRP. The error bars show the S.D. of three independent experiments.