FIGURE 2.

CANDIS interacts with the ADAM17 substrate IL-6R. A, schematic of a GPI-anchored membrane-proximal domain of human ADAM17 (MPD17plusCANDIS-GPI). The stalk region encompasses CANDIS as well as a PC and a myc tag (29). B, sequences of peptides that were coupled to Sepharose beads. The first peptide contains CANDIS, the second contains the corresponding sequence of ADAM10 (pA10), and the third peptide contains a randomized sequence of CANDIS (cp). C, Western blot (WB) analysis of the precipitation of the IL-6R by the peptides coupled to Sepharose beads. D, pulldown of human IL-1RII, murine TNF-α (FLAG-tagged), and human IL-6R by CANDIS or cp revealed that CANDIS interacts specifically with the IL-6R and IL-1RII but not with proTNF-α, as shown by Western blot analysis with the stated antibodies.