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. 2014 Apr 25;289(23):16374–16388. doi: 10.1074/jbc.M113.539601

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Confirmation of bone marrow cell repopulation. Bone marrow cells from WT 129J mice and Per1^ldc/Per2^ldc (129J background) mice were transplanted into lethally irradiated male WT C57BL/6J mice (at 5–6 weeks of age). After recovery for 4 weeks, chimeric mice (BMT-WT and BMT-Per1^ldc/Per2^ldc) were fed an HFD for 12 weeks. After the feeding regimen, genomic DNA was isolated from both isolated cells, and tissue samples were obtained from all chimeric mice and then subjected to PCR genotyping analyses to identify WT and targeted alleles (Per1, Per2) and confirm bone cell repopulation. The amplified band of smaller size (∼235 bp, top panel) signifies the Per1 targeted alleles, whereas the PCR product of larger size (∼403 bp, bottom panel) denotes the Per2 targeted alleles in bone marrow cells (A), BMDM (B), adipose tissue SVC (C), adipose tissue (D), and liver (E).