FIGURE 1.
Conductance-voltage relationship of KvAP in variable lipid conditions. KvAP channels were reconstituted in small unilamellar vesicles of DPhPC (black), POPE:POPG, 3:1 (blue), DOTAP (pink), POPC:POPE:POPS, 5:3:2 (green), and POePC (orange) and fused to a pure DPhPC planar lipid bilayer (A), a DOTAP:DPhPC, 2:1, bilayer (B), and a pure DOTAP bilayer (C). Voltage ranged from −100 to + 100 mV with 10 mV increments. Holding potential was kept at −100 mV. A single Boltzmann function was fit to the data (solid lines) and the activation parameters V½ and VS are presented in Table 1. The number of assays for each condition ranged from n = 2 to 7, error bars are S.D. For each bilayer condition, current traces are displayed for vesicles of DPhPC, POPE:POPG, 3:1, and DOTAP (from left to right). D, structures of DPhPC, DOTAP, POPG, POPE, POePC, and POPS.