FIGURE 8.

Temperature dependence of the binding intensities of mAb F77 and anti-I Ma. A and B, binding intensities elicited in microarray analyses of mAb F77, 1.0 μg/ml (A) and 0.1 μg/ml (B) at 4, 20, and 37 °C with the four branched human blood group H, A, and B glycolipids, H₃, Ad, BIII, and BIV. C, binding intensities elicited in microarray analyses of anti-I Ma (1:100) at 4, 20, and 37 °C with NGLs of the chemically synthesized oligosaccharides designated Orsay-1, -6, -7 and the B-like decaosylglyceramide. The results are the means of fluorescence intensities of duplicate spots printed at 5 fmol. The probe sequences of H₃, Ad, BIII, BIV, and B-like deca- are given in Table 2. The oligosaccharide sequences of Orsay-1, -6, and -7 are as follows: Orsay-1, Galβ-4GlcNAcβ-6Gal; Orsay-6 Galβ-4GlcNAcβ-6(Galβ-4GlcNAcβ-3)Gal; and Orsay-7 Galβ-4GlcNAcβ-6(Galβ-3GlcNAcβ-3)Gal.