FIGURE 3.

Loss of GLI1 increases tumor burden. A, representative ultrasound images of WT, KPC, and GKO/KPC mice captured at day 25 after birth. Developing pancreatic tumors (T), spleen (S), kidney (K), and normal pancreas (P). B, tumors measured by three-dimensional mode ultrasound show a lower tumor volume in KPC mice compared with the GKO/KPC animals at 25 days. C, similarly, GKO/KPC average body weight was lower compared with the KPC mice at 30 days after birth. D, GKO/KPC average pancreas weight was significantly higher. E, GKO/KPC mice have higher levels of LDH compared with KPC. F, CD4⁺/CD8⁺ T-cell ratio in GKO/KPC is significantly decreased compared with KPC. G, WT and GKO (no mutated kras) CD4⁺/CD8⁺ subpopulations in the spleen, mesenteric lymph nodes (MLN), or thymus.