TABLE 1.

Statistical parameters for the NADH-coupled and malachite green assays used in chemical screen for plasmodium Kinesin-5 inhibitors

Z-Factor, signal/noise, and signal/background ratios were for the basal assay condition, in the absence of microtubules. S/N, signal/noise; S/B, signal/background.

Screen assay	Z-Factor	S/N	S/B	Mean signal	Mean background	n
NADH-coupled
Day 1	0.65	26	27	0.18 ± 0.01	0.007 ± 0.005	20
Day 2	0.64	18	17	0.18 ± 0.01	0.011 ± 0.010	20
Cumulative	0.64	21	19	0.18 ± 0.01	0.009 ± 0.008	40
Malachite green
Day 3	0.68	33	44	0.17 ± 0.01	0.004 ± 0.005	40
Day 4	0.64	25	68	0.17 ± 0.01	0.003 ± 0.003	40
Cumulative	0.66	28	53	0.17 ± 0.01	0.003 ± 0.006	80