Fig. 2.

Representative results obtained with iTRAQ and SRM/MRM in the discovery and confirmation steps. A, The iTRAQ and SRM/MRM data for ITGA5, GPRC5A, PDGFRB, and TFRC are shown. P, polyp. C, cancer without metastasis. Cm, cancer with metastasis. Area ratio, the ratio of the peak area of the endogenous peptide to that of the SI peptide. Assays were constructed to measure two distinct peptides per-protein listed in supplemental Table S5 and that the individual assays for each of the two peptides are labeled SRM-1 and SRM-2. B, Western blotting analysis of polyps and cancer with and without metastasis using an anti-ITGA5 antibody.