Fig. 4.

Validation of the biomarker candidate C8orf55. A, The iTRAQ and SRM/MRM data of C8orf55. P, polyp. C, cancer without metastasis. Cm, cancer with metastasis. Area ratio, the ratio of the peak area of the endogenous peptide to that of the SI peptide. Assays were constructed to measure two distinct peptides per-protein listed in supplemental Table S5 and that the individual assays for each of the two peptides are labeled SRM-1 and SRM-2. B, Western blotting analysis of polyps and cancer with and without metastasis using an anti-C8orf55 antibody. C, Immunohistochemical staining of colorectal cancer tissue using an anti-C8orf55 antibody. Left panel: lower magnification. Right panel: higher magnification. The arrowhead shows areas of stained tumor cells and the arrow shows normal colon epithelial glands. Bar, 100 μm.