Table IV. SRM/MRM analysis of biomarker candidate proteins. p values were calculated by t-test. C/P, average ratio of cancer without metastasis to polyps. Cm/C, average ratio of cancer with metastasis to cancer without metastasis. Cm/P, average ratio of cancer with metastasis to polyps.

Gene name	C/P	p Value	Cm/C	p Value	Cm/P	p Value
FAP	1.59	0.515	2.21	0.052	3.52	0.198
GPRC5A	4.31	0.040	1.30	0.514	5.59	<0.01
CEACAM6	13.41	0.096	0.87	0.822	11.61	<0.01
LRRC15	2.51	0.084	1.83	0.237	4.59	0.037
PRTN3	2.68	0.014	1.67	0.098	4.47	<0.01
CEACAM5	7.29	0.044	0.85	0.737	6.22	<0.01
ITGA11	1.90	0.019	0.82	0.408	1.55	0.066
BST1	1.93	0.012	1.84	0.064	3.55	<0.01
MMP2	1.18	0.601	1.11	0.761	1.30	0.396
ANTXR1	3.23	<0.01	1.08	0.818	3.48	<0.01
PXMP4	2.29	<0.01	0.80	0.385	1.82	0.025
EFEMP1	1.30	0.478	1.04	0.900	1.35	0.358
LTBP2	1.83	0.036	1.10	0.676	2.02	<0.01
SLC2A3	3.56	0.030	0.92	0.817	3.28	<0.01
ITGA5	1.83	<0.01	1.79	0.162	3.28	0.031
MFAP2	1.41	0.394	1.17	0.496	1.65	0.128
TMEM97	2.00	<0.01	0.83	0.411	1.67	0.064
ENPEP	3.83	<0.01	1.13	0.445	4.32	<0.01
CD177	1.17	0.581	1.42	0.224	1.66	0.144
C20orf108	1.23	0.368	0.94	0.823	1.16	0.560
PDGFRB	2.22	<0.01	1.00	0.995	2.22	<0.01
FAM73B	1.22	0.207	0.51	<0.01	0.62	0.013
TSPAN9	1.75	<0.01	0.99	0.968	1.74	<0.01
GPC6	1.89	0.072	1.20	0.614	2.26	0.044
GGT5	2.06	0.034	1.24	0.432	2.56	<0.01
C5AR1	1.48	0.120	1.49	0.167	2.21	0.016
FBN1	1.37	0.443	1.34	0.257	1.84	0.072
FBLN2	1.75	0.102	1.02	0.946	1.79	0.087
SIGMAR1	1.74	<0.01	0.98	0.914	1.71	0.013
MMP14	2.43	<0.01	1.00	0.988	2.42	<0.01
TFRC	2.32	0.018	1.01	0.973	2.35	0.027
MRC2	2.09	<0.01	1.13	0.631	2.36	<0.01
SPARC	2.49	<0.01	0.82	0.317	2.03	0.027
HSPB1	2.73	0.016	1.50	0.231	4.10	<0.01
C8orf55	1.92	<0.01	0.74	0.123	1.42	0.024
FCGR1A	2.47	<0.01	1.40	0.277	3.45	<0.01
THY1	2.14	<0.01	1.00	0.983	2.15	<0.01
TMEM41A	2.04	<0.01	0.90	0.593	1.84	<0.01
SLC4A2	2.41	<0.01	0.92	0.746	2.21	0.014
FCER1G	2.23	<0.01	0.97	0.888	2.17	<0.01
MME	5.21	0.058	0.97	0.959	5.05	0.058
CEACAM1	5.95	0.025	0.83	0.646	4.92	<0.01
REEP6	1.21	0.509	0.97	0.934	1.18	0.608
GCNT3	1.75	0.078	1.46	0.306	2.55	0.063
AGR3	0.20	<0.01	1.89	0.073	0.38	0.021
ENTPD2	1.11	0.800	0.88	0.778	0.98	0.942
CLCA1	0.17	0.022	1.32	0.739	0.22	0.019
FCGBP	0.22	<0.01	1.15	0.782	0.25	<0.01
B3GNT6	0.32	<0.01	1.48	0.359	0.48	0.036
MUC2	0.14	<0.01	2.02	0.279	0.29	0.013
TFF3	0.33		2.80		0.93
ANXA13	0.23	<0.01	1.41	0.259	0.32	<0.01
AKAP5	0.19	0.016	0.83	0.487	0.16	0.013
C16orf62	0.76	0.442	0.59		0.45
PRG2	0.34	0.018	1.10	0.744	0.38	0.021
KIAA1324	0.32	<0.01	1.13	0.657	0.36	<0.01
EPB41L3	0.55	0.060	0.67	0.142	0.37	<0.01
COL1A2	1.55	0.438	1.22	0.650	1.90	0.031
COL1A1	1.39	0.590	1.19	0.737	1.65	0.093
COL3A1	1.24	0.642	1.34	0.517	1.67	0.212
POSTN	0.90	0.687	1.94	0.018	1.75	0.033
NT5E	1.05	0.802	1.21	0.473	1.27	0.329
PEX13	1.73	<0.01	0.81	0.224	1.40	0.025
UBAC2	1.87	<0.01	0.95	0.834	1.78	0.044
TLCD1	2.13	<0.01	0.76	0.335	1.63	0.113
SERPIND1	1.57	0.018	1.21	0.371	1.90	<0.01
SLC2A1	2.57	0.175	1.18	0.758	3.03	0.051
FAM173A	1.18	0.433	0.81	0.259	0.96	0.860
TMEM45B	1.35	0.255	0.97	0.918	1.30	0.400