Fig. 1.

Characterization of immobilized E-3810. A, E-3810 structure, in free form and after immobilization on agarose resin. B, E-3810 maintains the ability to bind FGFR2 after immobilization on resin. Immunoblot analysis against FGFR2 in A2780 protein extract (input, IN), flow-through (FT), and eluates (EL) obtained by affinity chromatography with immobilized E-3810 (15 μl of resin slurry for 1 mg of extract). Non-derivatized agarose beads were used as a negative control (empty resin). FT contained proteins not retained by the E-3810 resin; EL contained proteins captured by the resin and subsequently eluted. C, increasing volumes of E-3810 resin captured increasing amounts of FGFR2. Immunoblot analysis against FGFR2 for IN, FT, and EL obtained from the affinity chromatography experiments performed by incubating 1 mg of protein extract with 3, 15, or 40 μl of E-3810 resin slurry.