Fig. 3.

Theoretical analysis of human splice-variant-specific peptides. Pie chart of the number of theoretical splice-variant-specific peptides (SVSPs). 146,818 trypsin-digested peptides and 74,798 LysC-digested peptides were splice variant specific, corresponding to 16,935 and 13,593 splice variants, respectively. 3072 splice variants in the database were from single-isoform genes, and these generated approximately half of the SVSPs. When all SVSPs are combined, 17,562 splice variants in the EVDB can be identified via MS, assuming the presence of all splice variants and 100% sequence coverage.