| Acceptance/usage for glycomics |
Widely used |
Rarely used |
Widely used |
Moderately used |
| Throughput |
Medium, approximately 50 samples per instrument per day |
(Very) high, multiplexing with up to 96 capillaries enables analysis of thousands of samples |
(Very) high, as measurement of a sample can be performed at a sub-minute time scale |
Medium, approximately 100 samples per day per instrument |
| Required expertise |
Medium |
Medium |
High |
Very high |
| Resolution |
High |
High |
Very high |
Very high |
| Isomer separation |
Good |
Very good |
None |
Some |
| Quantification |
Very good |
Good |
Medium |
Good |
| Costs of equipment |
Ca. Euro 40–70,000 |
Ca. Euro 100,000 for a 4-capillary instrument |
Ca. Euro 100–500,000 |
Euro 200–500,000 |
| Costs per sample in high throughput mode |
Rather high costs, mainly due to low throughput and costs of consumables |
Low costs per sample, due to low running costs and parallelization by multiplexing |
Low costs per sample due to high throughput per instrument |
Very high costs, mainly due to expensive equipment and low throughput per instrument |
| Main advantages for genetic and epidemiological studies |
Reliable quantification, robustness |
Less demanding in sample preparation, low costs, high robustness and high throughput, no sample carry over; reliable relative quantification, very sensitive (low LOD) |
Low cost and high throughput, site specific glycosylation analysis, sensitive, enables structural elucidation via fragmentation experiments |
Reliable quantification, site specific glycosylation analysis, sensitive, enables structural elucidation via fragmentation experiments |
| Main disadvantages for genetic and epidemiological studies |
Inability to perform site specific glycosylation analysis, relatively low throughput and high cost |
Inability to perform site specific glycosylation analysis, comparatively small database (to be enlarged) |
Less reliable quantification, loss of sialic acids |
Relatively high costs |
| Specific advantages for IgG glycosylation analysis |
Differentiation of galactosylation on 3- and 6-arms, accurate quantification of IgG sialylation |
Differentiation of galactosylation on 3- and 6-arms, accurate quantification of IgG sialylation |
Differentiation of glycans on different IgG subclasses, analysis of only Fc glycans |
Differentiation of glycans on different IgG subclasses, analysis of only Fc glycans, accurate quantification of IgG sialylation |
