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A free-dropping impacted-injury simulator was used to create a fracture on the middle shaft of mice tibia (A). Immediately after injury, an X-ray was taken to confirm complete fracture (B). A custom-made plexiglass restrainer was used to confine the position of mice during ultrasound scanning (C). A swept scanning was performed on the longitudinal axis of fractured tibia from distal to proximal leg (arrow).
