Multiple regressions showed several inflammatory factors were correlated to the increases of echogenicity in the mice after tibia fracture (*, p < .05). The contribution of different candidate variables in echogenicity was examined by the backward elimination of each variable or combinations. The adjusted R-square (Adj. R2) suggested the best combination to reflect the hyperechogenic ultrasonography in fractured bone was the positive cells in TUNEL and COX-2 immunostaining (underline Adj. R2 = 0.963).