(A and B) Nitrosylation levels of GAPDH (A) and nuclear translocation of GAPDH (B) in striatum were decreased in mice treated with cocaine and CGP3466B compared to cocaine treated mice. (C) Acetylation of GAPDH and p53, as well as p300-GAPDH association in mice treated with cocaine with or without treatment by CGP3466B. (D and E) Analysis of p53 binding to PUMA promoter (D) and c-fos and PUMA levels (E) in mice treated with cocaine with or without CGP3466B. *p < 0.01, n = 3, one-way ANOVA, mean ± SEM. (F) Analysis of cell death by TUNEL assay in striatum induced by cocaine with or without chronic treatment with CGP3466B in mice. *p < 0.01, n = 9, one-way ANOVA, mean ± SEM. (G) Balance and coordination of motor activity evaluated by rotarod analysis test in mice receiving cocaine with or without CGP3466B. #p < 0.05, ##p < 0.01, n = 9, two-way ANOVA, mean ± SEM. See also Figure S4.