Table 2.
Quantitative Real Time PCR Primers
| Gene | Forward | Reverse |
|---|---|---|
| Acsl1 | GGTACCACTGATGGTATTCG | CATGGTTCTGACATCGTCGT |
| Acsl3 | AACCTGTCAGTTCCAAACCG | GCCAATTATAGTGCCCCAGA |
| Acsl4 | TGCCAAAAGTGACCAGTCCTATG | TGTTACCAAACCAGTCTCGGGG |
| Acsl5 | TCCCAGCCCACCTCTGATGATGTG | ACAAACTGTCCCGCCGAATG |
| Acsl6 | AAAGGCTAAGAGACCGGAGC | GTCAGGAAAGGCTCAGTTGC |
| Anf | CCATATTGGAGCAAATCCTGTG | CGGCATCTTCTCCTCCAGGT |
| α-Ska | TTGACGTGTACATAGATTGACTCGTTT | TGGCTGGCTTTAATGCTTCA |
| Asns | GCGGCCTCCAACCGGTCTTGTC | GCTACAGGCGGACTGCAGGCA |
| Bnp | GGGAGAACACGGCATCATTG | ACAGCACCTTCAGGAGATCCA |
| Glut1 | CAATGAAGTTTGAGGTCCAGTTGG | TGTGGTGTCGCTGTTTGTTGTAG |
| Glut4 | AGCTCGTTCTACTAAGAGCAC | TCGTCATTGGCATTCTGGTTG |
| Gapdh | GGTGCTGAGTATGTCGTGGA | ACTGTGGTCATGAGCCCTTC |
| PLN | ATGACGACGATTCAAATCTCTTGG | CATGGGTTTGCAAAGTTAGGCATAA |
| SERCA1/2 | GACGAGTTTGGGGAGCAGCT | CAGGTGGTGATGACAGCAGG |
| Tubulin | ACCGAAGCTGAGAGCAACAT | CACCATTTACCCCCAATGAG |
Abbreviations: Acsl, acyl-CoA synthetase; Anf, atrial natriuretic factor; α-Ska, α-skeletal actin; Asns, asparagine synthetase; Bnp, B-type natriuretic peptide; Gapdh, glyceraldehyde 3-phosphate dehydrogenase; Glut, glucose transporter; PLN, phospholambin; SERCA, sarco/endoplasmic reticulum calcium ATPase