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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Cancer Res. 2014 Apr 22;74(11):3127–3136. doi: 10.1158/0008-5472.CAN-13-3213

Figure 3. R167Q mutation decreases VHL binding to elongin c, elongin B and CulII in 786-0 cells.

Figure 3

VHL-null 786-0 cells were infected to stably express the indicated proteins. Cells were cultured in complete medium and were lysed in 0.5% NP-40 buffer supplemented with protease inhibitors and phosphatase inhibitors. GFP-Trap elutes and total lysates were resolved on 10% SDS-polyacrylamide denaturing gels and analyzed with the indicated antibodies. Long exposure (LE) blots show weak bands. SE, short exposure.