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. 2014 Jun 4;90(6):1082–1086. doi: 10.4269/ajtmh.13-0329

Figure 1.

Figure 1.

Sensitivity of the PCR and extraction methods to detect Leishmania parasite in RDT or FP. In A (Hs = Homo sapiens), a human-specific cytb PCR product (330-bp band) is shown to detect human DNA in eight patients (lanes 1–8), L. major(Lm) limiting diluted samples (lanes 9–12), mouse blood (lanes 13 and 14), and distilled water (D2W, lane 15). In B, detection of L. major kDNA (620-bp band) in limiting dilution solutions of L. major plotted in the RDT kits or FP with a parasite density (parasites per microliter) of 6.2 × 103 (lane 1, RDT; lane 2, FP), 2 × 103 (lane 3, RDT; lane 4, FP), 1.8 × 102 (lane 5, RDT; lane 6, FP), 26 (lane 7, RDT; lane 8, FP), 0 (lane 9, RDT; lane 10, FP), and 0 (lane 11, RDT; lane 12, FP). Lanes 13 and 14 correspond for RDT- and FP-negative controls, respectively. Note that 0 parasites/μL means that parasites were not detected under Giemsa staining microscopy. The M lanes stand for 100-bp molecular marker.