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. Author manuscript; available in PMC: 2014 Jun 6.
Published in final edited form as: Dev Cell. 2012 Mar 13;22(3):489–500. doi: 10.1016/j.devcel.2012.02.005

Figure 1. Alk1 Signaling Regulates Blood Vessel Morphogenesis.

Figure 1

(A–C) IsolectinB4 staining of wild-type P4 (A and B) and P6 (C) retinal vessels after treatment with control (A) or ALK1Fc adenovirus (B and C). Scale bar, 500 μm. (D) Quantification of vessel density in the area of retina covered by vessels in control adenovirus compared with ALK1Fc adenovirus at P6 (n = 7 mice/group). (E–L) P12 arterial vascular patterns shown by blue latex dye injected into the left ventricle of control (E–H) and ALK1Fc (I–L) adenovirus-injected pups. (F), (H), (J), and (L) show the corresponding IsolectinB4 staining of retinal vessels. (G), (H), (K), and (L) are higher magnification pictures of boxes shown in (E), (F), (I), and (J). Note abnormal artery formation and hypervascularization in ALK1Fc-treated retinas (I–L). A, artery. Scale bars, 200 μm (E, F, I, and J) and 70 μm (G, H, K, and L). (M) SMA (red) and PECAM-1 (green) staining of P12 ear skin from ALK1Fc adenovirus-treated mice. Note the presence of an AV shunt (arrowhead). Scale bar, 100 μm.

(N and O) IsolectinB4 staining of wild-type P12 retinal vessels after treatment with control (N) or Bmp9 (O) adenovirus. Scale bar, 250 μm.

(P) Quantification of vessel density in control adenovirus compared with Bmp9 adenovirus at P12 (n = 5 mice/group).

All values are mean ± SEM. *p < 0.05; ***p < 0.005; Student's t test.