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. Author manuscript; available in PMC: 2014 Jun 6.
Published in final edited form as: Dev Cell. 2012 Mar 13;22(3):489–500. doi: 10.1016/j.devcel.2012.02.005

Figure 2. Effect of Notch and ALK1 Signaling on Endothelial Sprouting In Vitro and In Vivo.

Figure 2

(A) Representative images of HUVECs sprouting in a fibrin gel in the presence or absence of ALK1 or Notch agonists and antagonists. Scale bar, 75 μm. (B) Quantification of tube surface area. Graphs represent the average of three to five experiments. (C) Representative images and vascular area quantification of P5 retinas from wild-type or Dll4+/— pups that received intraperitoneal injections of control or Bmp9 blocking antibody. Scale bar, 120 μm.

(D) Quantification of vascular area at P5 (WT plus control antibody, n = 9 retinas; WT plus anti-Bmp9 antibody, n = 4 retinas; Dll4+/— plus control antibody, n = 9 retinas; Dll4+/— plus anti-Bmp9 antibody, n = 9 retinas).

(E) Representative images and quantification of tip cells of P5 retinas from pups injected with vehicle control or DAPT that received intraocular injections of PBS or BMP9. Scale bar, 36 μm.

(F) Quantification tip cells at the vascular front 16 hr after intraocular injection (n = 4 retinas for control PBS and BMP9; n = 6 retinas for DAPTPBS and BMP9).

All values are mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.005; Student’s t test.

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