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. Author manuscript; available in PMC: 2014 Jun 6.
Published in final edited form as: Dev Cell. 2012 Mar 13;22(3):489–500. doi: 10.1016/j.devcel.2012.02.005

Figure 5. ALK1 Signaling through SMAD and HEY Prevents Tip Cell Specification.

Figure 5

HUVECs transfected with siRNA were labeled with green or red fluorescent dyes, mixed, coated on microcarrier beads, and embedded in fibrin. (A, B, D, and E) Representative images of sprouts 4 days after siRNA transfection. Scale bar, 65 μm.

(C) Quantification of percentage of target siRNA-transfected cells at the tip position of sprouts. Sprouts on 25–30 beads were quantified per experiment. Graphs represent the average of three to four experiments. All values are mean ± SEM. *p < 0.05; Student's t test.

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