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. 2014 May 6;11(1):19. doi: 10.1186/1559-0275-11-19

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Copyright © 2014 Gao et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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SUR2 is a complex-glycosylated protein. (A) SUR2 topology and epitope positions for T1 and BNJ2. (B) De-glycosylation of SUR2 protein by PNGase F. T1 (1:2000) or BNJ-2 (1:1000) was used as the primary antibody. BNJ-2 was used as a negative control for T1 and a loading control. Secondary antibody was added at 1:10,000. (C) Treatment protocols for R, I and I-R. Grey arrows indicate endpoints when mouse hearts were harvested. (D) Representative Western blots using R-, I- or IR- treated WT LV samples. T1 was used as the primary antibody (1:2000). Secondary antibody was added at 1:10,000. n = 4. *, p < 0.05. The blot was stripped and re-probed with GAPDH (1:2000), and the density values were normalized to GAPDH.