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. 2014 May 6;11(1):19. doi: 10.1186/1559-0275-11-19

Figure 5.

Figure 5

Glycosylated SUR2 and estrogen regulation. (A) Representative co- immunoprecipitation results in WT LV samples from R-, I- and IR- groups. The R group was used as a reference control. 500 μg protein (combined from 5 independently handled mice) was immuno-precipitated by 7 μg anti-SUR2 (T1), followed by immunoblotting using anti-concanavalin A (ConA) (1:1000). (B) Representative DPM1 Western blots in WT LV samples. Anti-DPM1 was used as the primary antibody (1:500). Secondary antibody was added at 1:10,000. n = 4. *, p < 0.05. The blot was stripped and re-probed with GAPDH (1:2000), and the density values were normalized to GAPDH. (C) Estrogen (E2) or DMSO (vehicle) treatment in cultured COS1 cells co-expressing Kir6.2/SUR2. T1 was used as the primary antibody (1:2000). Secondary antibody was added at 1:10,000.