Table 3.

Kinetic and energetic parameters for anti-fluorescein 4-4-20 unbinding from fluorescein, from fits to the model of Evans-Ritchie,²¹ Hummer-Szabo²² and Dudko et al.²³ assuming a cusp shaped (ν=1/2) or a linear-cubic (ν=2/3) energy barrier.

Loading rate	Evans-Ritchie model		Hummer-Szabo model			Dudko-Hummer-Szabo model
						ν=1/2			ν=2/3
	τ0a	x‡b	τ0	x‡	ΔG‡c	τ0	x‡	ΔG‡	τ0	x‡	ΔG‡
6.1 pN·s−1	9.34	0.171	10.9	0.263	3.65	10.8	0.262	3.63	10.8	0.248	2.99
11.8 pN·s−1	7.13	0.176	10.5	0.318	4.14	10.1	0.311	4.09	10.8	0.308	3.54
24.7 pN·s−1	7.84	0.144	12.8	0.259	4.65	12.7	0.257	4.66	14.4	0.260	4.01

^aUnits are s

^bUnits are nm

^cΔG^‡ is in k_BT units.