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. 2014 Jun 6;5:211. doi: 10.3389/fphys.2014.00211

Figure 4.

Figure 4

qPCR on M. incognita chromatin that had been immuno-precipitated with various volumes (0–16 μl) of Anti-H3, Anti-H3K4Me3, and Anti-H4K20Me3 antibodies (1 μg/μl). The amount of target DNA recovered in the immune-precipitated fraction was quantified by calculating the percent input recovery (% IR) normalized with the percent input recovery obtained with the housekeeping gene as previously described by Cosseau et al. (2009).