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. 2014 May 22;5(5):e1247. doi: 10.1038/cddis.2014.222

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Copyright © 2014 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/

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SPAG5 knockdown efficiency in cervical cancer cell lines. (a) Western blot of siRNA specific to SPAG5 knockdown efficiency in SiHa cells (left), gradient analysis (middle); mRNA expression (right) was detected by qRT-PCR. (b) Transient knockdown reflected in levels of SPAG5 protein (left) and mRNA (right) in HeLa cells. (c) SPAG5 expression in shSPAG5 lines detected by western blotting (right) and qRT-PCR (left)