Figure 1.

Changes in organoid morphology after cell death induction. Crypts from murine small intestine were seeded into Matrigel and cultured for 72 h to obtain organoids. (a) Organoids were treated with different doses of mTNFα (0, 10, 30 and 100 ng/ml) overnight, and morphology of the crypts was assessed by light microscopy. (b) The relative number of organoids with altered morphology was counted for each dose of TNFα. At least 100 organoids per well were counted. Mean values ±S.D. of triplicates are shown. Data are representative for three independent experiments. **P<0.01 control versus 30 ng/ml TNFα; ***P<0.001 control versus 100 ng/ml TNFα. (c) Organoids were either treated overnight with cisplatin (10 μg/ml) or were UV irradiated (200 mJ), and the percentage of organoids with changed morphology was quantified using light microscopy. Mean values of three independent experiments ±S.D. are shown. *P<0.05 control versus UV-irradiated; **P<0.01 control versus cisplatin-treated