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. 2014 May 22;5(5):e1242. doi: 10.1038/cddis.2014.224

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Copyright © 2014 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

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Tip30 deletion enhanced the mammosphere and colony-forming capacity of mammary cells. (a) Freshly isolated mammary cells from mice at 5 months of age were plated in ultra-low attachment plates (5000 cells per well) with serum-free DMEM/F12 medium supplemented with growth factors. After 10 days in culture, mammosphere's number and size were calculated as shown in histograms. Only mammospheres exceeding 60 μm in diameter were counted. Error bars represent the mean±S.E.M. of three mice per group. (b) Freshly isolated mammary cells from mice were plated in Matrigel-coated 48-well plates (2000 cells per well). Colonies were cultured for 9 days and then photographed. The histograms show colony number and size. The results are the mean±S.E.M. of three mice per group