Figure 4.

Tip30 deletion promotes expansion of the MaSC-enriched and progenitor-enriched subpopulation in mammary glands by flow cytometry. (a) Flow cytometry dot plots of primary mouse mammary cells isolated from 5-month-old virgin mice and stained with antibodies for CD24 and CD49f. (b) The histograms show the percentage of mammary repopulating units and mammary colony-forming cells of subpopulation in mammary cells. Error bars represent the mean±S.E.M. of three mice per group. (c) CD24^highCD49f⁺ subpopulation was further analyzed by Sca1. Sca1⁺CD24^highCD49f⁺ or Sca1⁻CD24^highCD49f⁺ cells sorted from Tip30^−/− female mice (n=6) were further analyzed by immunonocytochemistry with anti-ERα antibodies. About 77% of Sca1⁺CD24^highCD49f⁺ and ∼26% Sca1⁻CD24highCD49f⁺ cells were stained positive for ERα. (d) The histogram shows percentage of Sca1⁺ cells in CD24^highCD49f⁺ subpopulation. Error bars represent the mean±S.E.M. of three mice per group