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. 2014 May 22;5(5):e1242. doi: 10.1038/cddis.2014.224

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Copyright © 2014 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

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Tip30 deletion predisposed mammary luminal progenitors to commitment of ER⁺ luminal cell differentiation. (a) Representative immunofluorescent staining of ERα and PR in colonies formed by progenitor cells from Tip30^−/− and Tip30^+/+ mammary gland. Mammary epithelial cells isolated from 5-month-old virgin mice were processed to single-cell suspensions and stained with CD24 and CD49f. Progenitor cell-enriched population (CD24^highCD49f⁺) was sorted out and plated in Matrigel-coated 48-well plates. After cultured for 9 days, colonies were Immuno stained for ERα and PR-A. Scale bar: 50 μm. (b) The histogram shows the percentage of ERα-positive and PR-A-positive cells in the colonies. Error bars represent the mean±S.E.M. of three mice per group. (c) Representative immunofluorescent staining of ERα and PR-A in mammary glands from 5-month-old Tip30^−/− and Tip30^+/+ mice. Scale bar: 20 μm. (d) The histogram shows the percentage of ERα-positive and PR-A-positive cells in mammary glands. The bar graphs denote the mean±S.E.M. of three animals per group. Scale bar: 20 μm