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. 2014 May 29;5(5):e1263. doi: 10.1038/cddis.2014.206

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Copyright © 2014 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Knockdown of Elf3 suppresses invasion and proliferation and induces apoptosis and cell cycle arrest of colorectal cancer cells. The HCT116 and SW1116 cells were transient transfected with Elf3 siRNA or control siRNA for subsequent analyses. Data in all statistical plots represent means±S.D. (a, b) Flow cytometry assay of HCT116 cells treated by Elf3 siRNA. The knockdown of Elf3 induced significant increase of cells in sub-G1 and G1 phases as compared with control siRNA. The statistics of cell cycle distribution are shown in (a) and representative FACS images are shown in (b). (c, d) Apoptosis of HCT116 and SW1116 cells induced by Elf3 knockdown. Flow cytometric assay based on phycoerythrin (PE)-conjugated annexin V staining showed increased apoptosis of HCT116 and SW1116 cells treated by Elf3 siRNA. Statistics based on 3 independent experiments is shown in (c), and representative FACS images are shown in (d). (e, f) Proliferation curves of HCT116 (e) and SW1116 cells (f) as determined by CCK-8 assay. Significant difference in cell viability was found 72 h after cell plating (P<0.001, two-sided t-test). (g, h) Knockdown of Elf3 suppressed migration of CRC cells. The HCT116 and SW1116 cells were transfected by Elf3 siRNAs, and transwell assay was applied to quantify cell migration ability. Statistical result based on three independent experiments is indicated in (g), and representative transwell cell staining images are shown in (h)