Table 1. Effect of rasfonin on the kinases activity (%).
| Protein kinase | Activity (% control) |
|---|---|
| Control | 100±2 |
| EGFR | 113±3 |
| FAK | 102±0 |
| FGFR3 | 97±4 |
| Flt3 | 98±3 |
| Met | 113±0 |
| PDGFRα | 105±1 |
| PDGFRβ | 108±9 |
| PKBα | 113±3 |
| PKBβ | 98±2 |
| PKBγ | 117±6 |
| c-RAF | 86±6 |
| MEK1 | 90±2 |
| IKKα | 114±11 |
| JNK1α1 | 93±2 |
| JNK2α2 | 116±3 |
| mTOR | 112±13 |
| p70S6K | 149±9 |
| Ros | 106±2 |
| PI3 kinase (p110a/p85a) | 99±4 |
Effects of rasfonin on EGFR family kinase activities. Kinase activities were measured using [32P] radiometric protein kinase assays after treatment with 5 μM rasfonin and incubation for 40 min. The results were expressed as percentage c.p.m. versus control (% control) defined as ((sample−mean no enzyme)/(mean plus enzyme−mean no enzyme)) × 100.