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. 2014 May 1;5(5):e1202. doi: 10.1038/cddis.2014.173

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Copyright © 2014 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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NGF-induced removal of active caspase-3 does not involve macroautophagy. PC12 cells were stably transduced with a lentiviral vector encoding functionally inactive Atg4 (DN-Atg4). (a) PC12 cells were treated with 50 μM CQ, and the lipidation state of LC3 protein was analysed by western blotting with anti-LC3 antibody. Actin was used as a loading control. (b) Parental PC12 cells, PC12 cells transduced with empty vector (Neo) and those transduced with DN-Atg4 were treated with 1.5 μM TG for 20 h followed by NGF cells. The cells were harvested at 20–26 h as indicated, and the levels of the p17 subunit were analysed by western blotting using the cleaved-caspase-3 antibody. Actin was used as a loading control. Images are representative of three independent experiments