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. 2014 May 8;5(5):e1223. doi: 10.1038/cddis.2014.188

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Copyright © 2014 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Immunoblot analysis of UCN-01 targets. (a) GSC lines selected from each sensitivity group were exposed to increasing doses of UCN-01 and the phosphorylation levels of PKC, PDK1, CDC25C, and CDK1 (CDC2) were evaluated at 72 h. Representative blots are shown for three GSC lines (Supplementary Figure S4 displays the blots performed on the other tested GSC lines in each sensitivity group). (b) Normalized intensities of the immunoblot bands from each antibody tested are plotted for all three GSC lines in each sensitivity group as percentage over the untreated control. No statistically significant difference between sensitivity groups at various UCN-01 concentrations was found after two-way ANOVA and Bonferroni post test