Figure 5.

NHS-dependent DNA degradation in apoptotic Jurkat cells. (a) Jurkat cells were cultured in UC medium in the presence of 20 μM oxaliplatin for 48 h and stained with 7AAD. Then 25% NHS was added and the 7AAD signal was measured at different points in time (0–3 h). Then the 7AAD signal was measured by flow cytometry. The gray line represents the result for cells incubated at 0 °C. The broken line indicates the result for cells incubated at 37 °C in heat-inactivated NHS. The Roman numerals indicate the position of populations I, II and III. (b) Jurkat cells were treated as in a, then incubated for 3 h in serum-free UC medium (dotted line), 25% NHS (gray line) or 25% NHS supplemented with the DNase I inhibitor G-actin (black line). (c) Jurkat cells were cultured in UC medium, heat shock treated and stained with 7AAD. Then 25% NHS was added and the 7AAD signal was measured at different time points (0–3 h)