Figure 8.
MiR-374a and miR-548b regulate tumorigenesis of gefitinib resistance lung cancer cells in vivo by modulating apoptosis. (a and c) Representative image for tumor growth is shown. Nude mice were subcutaneously injected with 2 × 106 HCC827-Gef-miRZip-374a and HCC827-Gef-miRZip-control cells, or HCC827-Gef-p-miR-548b and HCC827-Gef-p-miR-control cells. (b and d) Determination of the tumor growth. Tumor volume was calculated every week after injection. Data are mean+S.D.s of three independent experiments. *P<005. (e and f) The representative images of TUNEL staining. The apoptotic percentage was significantly increased in tumor tissues derived from HCC827-Gef-miRZip-374a and HCC827-Gef-p-miR-548b tumors compared with those from the control cells. (g) The quantification of TUNEL-positive cells from different fields in the tissue section have been showed in the graph. *P<0.05