Figure 5.

Hep3B cells cultured in (a) serum-free or (b) 1% serum medium were pre-transfected with mTOR siRNA (30 nM) for 24 h, then subsequent treatment of the PAR2 agonist (200 ng/ml) for 24 h was performed and the cell survival rates were measured by cell counting kit (CCK-8). All results are expressed as the mean±S.D. from three independent experiments. *P<0.05, **P<0.01 all compared with control