Figure 4.

The role of NF-κB in radiation-induced metabolic alterations in securin-knockdown MDA-MB-231-2A cells. (a) MDA-MB-231-2A cells were exposed to 6 Gy radiation followed by various recovery periods. The protein expression was examined by western blot analyses. (b–d) MDA-MB-231-2A cells were pretreated with 5–20 μM compound C (b), 5–20 mM DCA (c) or 1–10 μM BMS-345511 (d) for 2 h and then exposed to 6 Gy radiation. After an 8-h recovery period, the protein expression was examined by western blot analyses. (e) MDA-MB-231-2A cells were pretreated with 5 or 10 μM BMS-345511 for 2 h and then exposed to 6 Gy radiation. After a 24-h recovery period, the lactate concentration in the culture medium was examined by protocols described in the Materials and Methods section. **P<0.01 indicates significant differences between control and irradiated cells. ^##P<0.01 indicates significant differences between inhibitor-treated and untreated cells. (f) MDA-MB-231-2A cells were pretreated with 1–10 μM BMS-345511 for 2 h, and then exposed to 6 Gy radiation. After an 8-h recovery period, the protein expression was examined by western blot analyses