Figure 5.

WIF1 reduces salivary gland tumor stemness and the expression of several genes with critical roles in stem cell self-renewal and pluripotency. (a) PA116 and CaExPA79 cells were transfected with vector or WIF1 and 48 h later sorted by FACS-based on aldehyde dehydrogenase (ALDH) activity. Data are the mean±S.D., n=3. *P<0.001. (b) PA116 (left panel) and CaExPA79 (right panel) cells transfected with vector or WIF1 were assessed for their ability to form spheroids in soft agar. The number of spheroids per well was counted on day 5 and 14. Data are the mean±S.D., n=3. *P<0.001. (c) Representative photo images of PA116 (left panel) and CaExPA79 (right panel) cells at day 5 (top panel) and 14 (bottom panel). Original magnification, 400X. (d) Total RNA isolated from CaExPA79 cells transfected with WIF1 was subjected to real-time RT-PCR and relative mRNA expression of OCT4, c-MYC, WNT3A, TCF4, MYB and c-KIT was assessed. The transcripts were normalized with β-actin. Data are the mean±S.D., n=3. *P<0.0001