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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: J Immunol. 2013 Sep 25;191(9):4804–4817. doi: 10.4049/jimmunol.1301307

Figure 1. GM35 binds to the O-linked glycan sLea.

Figure 1

T84 or HT29 IECs were pretreated with (A) 5μg/ml Tunicamycin or (B) 4mM Benzyl-GalNAc before whole cell lysis and western blotting with GM35. Data represent N=3 immunoblots. (C) The glycans recognized by GM35, including glycan number, glycan structure, glycan name and relative fluorescence units (RFU) intensity are depicted with sLex is included as a negative binding control. (D) Protein lysates from T84 or HT29 IECs were treated with 50U neuraminidase before whole cell lysis and western blotting with GM35. Data represent N=3 immunoblots.