(A) Schematic representation of the glycosyltransferases responsible for the biosynthesis of sLea. (B) RNA was isolated from indicated IECs and analyzed by real-time PCR for levels of expression of ST3Gal3, Fut3 and Fut6. Results are depicted as fold change relative to Caco2 IECs and normalized for expression of the housekeeping gene GAPDH. N=3-6, significance was defined at p<0.05 (*, p<0.05; **, p<0.01; ***, p<0.001). (C) Following epithelial cell lysis, equal amounts of protein lysates from indicted IECs were subjected to SDS-PAGE under reducing conditions and immunoblotted with indicated antibodies. Data represent N=3 immunoblots.