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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: J Immunol. 2013 Sep 25;191(9):4804–4817. doi: 10.4049/jimmunol.1301307

Figure 4. Differential expression of glycosyltransferases in human IECs.

Figure 4

(A) Schematic representation of the glycosyltransferases responsible for the biosynthesis of sLea. (B) RNA was isolated from indicated IECs and analyzed by real-time PCR for levels of expression of ST3Gal3, Fut3 and Fut6. Results are depicted as fold change relative to Caco2 IECs and normalized for expression of the housekeeping gene GAPDH. N=3-6, significance was defined at p<0.05 (*, p<0.05; **, p<0.01; ***, p<0.001). (C) Following epithelial cell lysis, equal amounts of protein lysates from indicted IECs were subjected to SDS-PAGE under reducing conditions and immunoblotted with indicated antibodies. Data represent N=3 immunoblots.