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. Author manuscript; available in PMC: 2015 Mar 20.
Published in final edited form as: Mol Cell. 2014 Mar 6;53(6):993–1004. doi: 10.1016/j.molcel.2014.01.032

Figure 1. pRB loss compromises chromatid cohesion and compaction during S phase and mitosis.

Figure 1

A&B) Fluorescence in situ hybridization (FISH) with probes specific for 16p13 (green) and 16q22 (red) were used to quantify mitotic cohesion (increased distance between replicated foci of the same color) in control and pRB-depleted cells +/− prolonged mitotic arrest induced by 4 hr colcemid treatment (+ colc). pRB loss decreased mitotic cohesion, a change enhanced by prolonged mitotic delay. C-E) Measures of cohesion and compaction (distance between red and green foci) in interphase cells show significant decreases in chromatin cohesion (D) and compaction (E) as early as S phase following pRB loss. S phase progression is required for decreased compaction following pRB loss (0.2mM Thymidine). F) Cell cycle changes in compaction following depletion of cohesin components (Rad21, SMC3), but not condensin (CAPD3) closely mirrored the effects seen following pRB depletion. **p<0.01; ***p<0.001. See also Figures S1&S2.