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. Author manuscript; available in PMC: 2015 Mar 20.
Published in final edited form as: Mol Cell. 2014 Mar 6;53(6):993–1004. doi: 10.1016/j.molcel.2014.01.032

Figure 4. Promoting cohesion suppresses S phase defects in replication and chromatin structure resulting from pRB loss.

Figure 4

DNA fiber combing assays were performed and fiber lengths measured for control- and pRB- depleted cells alone or following co-depletion of Wapl or addition of nucleosides (+nuc). A) Loss of pRB promoted replication defects and resulted in shorter fibers (p<0.0001). B) Co-depletion of Wapl, or C) supplementation of nucleosides in pRBdepleted cells suppressed replication defects and allowed for longer fiber formation (p<0.0001 v siRB for both). D) Representative fibers from each condition are shown. FISH based assays to measure E) chromatin compaction and F) chromatin cohesion revealed that co-depletion of Wapl and nucleoside addition both suppressed compaction and cohesion defects during S phase in pRB-depleted cells, while Mad2 co-depletion did not. * p<0.05; **p<0.01. See also Figure S5.