A & B) RB1 null osteosarcoma (SAOS2) cells were fixed and stained for tubulin, Hec1, and DAPI following re-expression of pRB with a tetracycline regulated construct (tetRB), depletion of Wapl, and/or addition of nucleosides. All three conditions induced a moderate, but significant increase in mitotic sister chromatid cohesion, C) reduced anaphase defects and suppressed replication defects (Figure S5). Combined depletion of Wapl with nucleoside addition provided no additional suppression of cohesion or anaphase defects, but does enhance D) suppression of replication defects (siScr: 3.22µm; siWapl+nucl: 4.969µm; p<0.0001; and Figure S5). E) FISH-based measures of chromosome segregation errors in recently divided daughter cells show that depletion of Wapl and addition of nucleosides decreased CIN in SAOS2 cells. *p<0.05