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. 2014 May 30;9:2727–2739. doi: 10.2147/IJN.S60014

Figure 2.

Figure 2

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE; Coomassie blue staining) analysis of VP6 fusion proteins expression in Escherichia coli. (A) Lane 1, protein marker; lane 2, soluble cell extract from the uninduced culture transformed with pET15b-VP6; lane 3, soluble cell extract of the induced culture transformed with pET15b-VP6; lane 4, soluble cell extract from the uninduced culture transformed with pTRX-VP6; lane 5, soluble cell extract of the induced culture transformed with pTRX-VP6; lane 6, soluble cell extract of the uninduced culture transformed with pET-SUMO-VP6; lane 7, soluble cell extract of the induced culture transformed with pET-SUMO-VP6. (B) Lane 1, protein marker; lane 2, insoluble cell extract from the uninduced culture transformed with pET15b-VP6; lane 3, insoluble cell extract of the induced culture transformed with pET15b-VP6; lane 4, insoluble cell extract from the uninduced culture transformed with pTRX-VP6; lane 5, insoluble cell extract of the induced culture transformed with pTRX-VP6; lane 6, insoluble cell extract of the uninduced culture transformed with pET-SUMO-VP6; lane 7, insoluble cell extract of the induced culture transformed with pET-SUMO-VP6. The position of each fusion protein in the SDS-PAGE gel is marked by a black arrow.

Abbreviations: SUMO, small ubiquitin-like modifier; TRX, thioredoxin.