Table 1.

Primers used in this study

Vector	Primer pair	Restriction site
pET15-b	Fw 5′-CATACATATGGAGGTTCTGTATTCATTGT-3′ Rev 5′-CATACTCGAGCTTAATCAACATGCTTCTG-3′	NdeI XhoI
pET-SUMO	Fw 5′-TCACTTAATCAACATGCTTCTGAT-3′ Rev 5′-TCACTTAATCAACATGCTTCTGAT-3′
pTRX	Fw 5′-CATAGGATCCGAGGTTCTGTATTCATTGT-3′ Rev 5′-CATACTCGAGCTTAATCAACATGCTTCTG-3′	BamHI XhoI

Note: Underlined bases correspond to the restriction sites reported in the last column of the table.

Abbreviations: Fw, forward; SUMO, small ubiquitin-like modifier; PCR, polymerase chain reaction; Rev, reverse; TRX, thioredoxin.