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. 2014 Mar 31;592(Pt 11):2343–2355. doi: 10.1113/jphysiol.2014.271445

Figure 2. Exposure to G418 in F1 induces widespread changes in the composition of maternal RNA in the early F2 embryos.

Figure 2

A, scatter plot of RPKM values of maternal RNA (0–2 h AEL) in F2 embryos of exposed versus non-exposed F1 flies. Each dot represents the corresponding transcription level for a particular gene in each case. Red lines indicate a difference of 1.5-fold between RPKM values. RPKM, reads per kilobase of exon model per million mapped reads. B, same as A for RPKM values measured in the zygotic stage (8–10 h AEL). C, number of genes changed (left) and distributions of differences (right) in levels of maternal RNA (0–2 h AEL) in F2 embryos of exposed versus non-exposed flies. Fractions of genes that were up- and down-regulated over 1.5-fold are labelled red and blue, respectively. Values are based on average measurements in two biological replicates. D, same as C for changes observed in the zygotic stage (8–10 h AEL). E, examples of enrichments of specific Gene Ontology (GO) annotations in the groups of genes that were up- (red) and down- (blue) regulated in the maternal stage. P-values, corrected for multiple testing using the DAVID web tool with Benjamini correction (Huang da et al. 2009b; Huang da et al. 2009a).