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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Nat Chem Biol. 2014 Apr 6;10(5):386–391. doi: 10.1038/nchembio.1498

Figure 4. Crystal structures of OSH55.4_1 bound to Diispropyl fluoroposphate (DFP).

Figure 4

(a) Crystal Structure of the apo form of OSH55.4_1 with a citrate molecule (white) from the crystallization buffer cocktail making extensive interactions with active site residues. (b) Crystal structure of OSH55.4_1 covalently modified at Ser151 with FPyne (pink). (c) Crystal structure of OSH55.4_1 covalently modified at Ser151 with DFP (red and blue). (d) Simulated annealing omit map around Ser151 (fo-fc). Density is clearly evident on the serine but the isopropyl groups of DFP are not distinguishable probably due to free rotation about the P-C bond.